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KMID : 0378019750180040439
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New Medical Journal
1975 Volume.18 No. 4 p.439 ~ p.446
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Liver Transplantation In the Rat-Nucleic Acid Contents and Guanine Aminohydrolase Activity(GDA) in Isogeneic Auxiliary Liver Graft Supplied by Portal Venous Blood alone
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Abstract
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In order to assess portal venous blood alone can maintain the function of the heterotopic auxiliary liver graft in rats and study the nature of the graft survival, guanine aminohydrolase (GDA) and GPT activities as well as nucleic acids were examined in the graft.
Isogeneic liver transplantation was performed on Lewis rats, weighing 250 to 300 gms by the suture technique of Lee´s original method, but only portal blood flow was induced to the graft, and the bile duct was introduced into the upper jejunum.
All recipients in these experiments were sacrificed 1, 2, 6,12 and 14 days subsequent to the surgical procedure, and biochemical and histological studies were performed in the graft. The following results were obtained.
I. Biochemical Studies
1. DNA contents were increased, and the peak was on the postoperative 6 days.
2. GPT activities in the graft showed marked decrease and about half as much as the control. 3. GDA activities were increased and these findings were remarkable on the postoperative 12 days.
11. Histological Studies 1. During observation period the graft showed progressive weight increaseand about two times of the initial weight to the postoperative 6 days.
2. Some of the grafts revealed findings of outflow-block, inflammation or infection.
Discussion
The increase of DNA contents in the graft may mean the hyperplasia of the hepatic cells, which is also supported by the increase in the weight of the graft. The increase of-GDA activities can be interpreted as acceleration of purine metabolism in consequence of the hyperplasia, and the fact that the peak of GDA activities appeared on the postoperative 12 days may tell the time requirement for the enzyme induction following the increase of DNA due to hyperplasia. The remarkable decrease of GPT activities in the graft may be considered as the result of decreased liver function due to damage or functional inefficiency due to immaturity of the hepatic cells.
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